Confocal laser scanning microscopy showing LD revealed by the Nile Red stain technique in SkMC uninfected and infected with T. gondii . (A) The LD (arrow) in uninfected SkMC (arrowhead) stained in red appears distributed throughout the cell cytoplasm and concentrated around the nucleus (N) (thin arrows). (B and C) After 24 h SkMC infection by T. gondii a clear association of several LD (thin arrows) can be seen with the PV (thick arrows). Fibroblasts play little LD labeling (*). Bars: A = 50 μm; B and C = 20 μm.