Confocal Laser Scanning Microscopy showing LD revealed by BODIPY in uninfected and T. gondii -infected SkMC for different periods of interaction. (A) Control - interferential microscopy revealing the profile of uninfected culture with the presence of myoblasts (double arrows), multinucleated myotubes (arrows) and fibroblasts (arrowhead). (B) Merge showing double marking for actin cytoskeleton and nuclei SkMC (arrows) in red and LD in green. Note discrete distribution of LD across culture. (C) Interferential microscopy showing parasites in T. gondii-infected SkMC (thick arrows) after 6 h of the interaction. (D) Merge showing actin cytoskeleton and nuclei in red and LD in green in T. gondii-infected SkMC. All bars = 20 μm.