Skip to main content
Figure 2 | Parasites & Vectors

Figure 2

From: Acanthamoeba and bacteria produce antimicrobials to target their counterpart

Figure 2

Anti-Acanthamoebic activities of conditioned media of Enterobacter sp. and E. coli K1 are resistant to pronase digestion and smaller than 5 kDa in molecular mass. (A) The conditioned media of Enterobacter sp. and E. coli K1 were prepared in PYG. The conditioned media were diluted in PYG in a 1:1 ratio and treated with 1 mg per mL of Pronase at 37°C for 4 h. Next, A. castellanii cells (105) were incubated with Pronase-treated and untreated conditioned media at 30°C for 48 h. After incubation, A. castellanii were counted using a haemocytometer. The initial inoculum of 105A. castellanii was used as a baseline, which increased to over 8-fold in PYG alone. Note that Pronase-treatment had no effect on growth inhibitory effects of conditioned media. (B) Enterobacter sp. and E. coli K1 conditioned media were filtered through 10 kDa and 5 kDa MWCO Spin-X UF. The eluate and neat conditioned media (unfiltered) were incubated with A. castellanii cells (105) for 48 h at 30°C. After incubation, A. castellanii were counted using a haemocytometer. The initial inoculum of 105A. castellanii was used as a baseline, which increased to over 8-fold in PYG alone. Note that eluate from 5 kDa filters exhibited growth inhibitory effects at levels similar to neat conditioned media. The data are presented as the mean ± standard error of three independent experiments performed in duplicate.

Back to article page