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Table 1 Acanthamoeba conditioned medium (ACM) exhibited selective antibacterial properties

From: Acanthamoeba and bacteria produce antimicrobials to target their counterpart

Bacteria + Acanthamoeba conditioned medium (ACM) CFU remaininga(Bacteria + ACM) Bacterial kill (%)
Methicillin-resistant Staphylococcus aureus (MRSA, 10 μL) + ACM (190 μL) 0 100
Acinetobacter (10 μL) + ACM (190 μL) 1 × 1010 0
Pseudomonas aeruginosa (10 μL) + ACM (190 μL) 6 × 109 0
Vancomycin resistant Enterococcus faecalis (VRE, 10 μL) + ACM (190 μL) 3.75 × 105 8.12 ± 9.8
MRSA (10 μL) + heated ACM (190 μL) 0 100
MRSA (10 μL) + heated ACM (180 μL) + PYG (10 μL) 6.65 × 104 93.3 ± 9.9
MRSA (10 μL) + heated ACM (140 μL) + PYG (50 μL) 1.55 × 107 0
MRSA (10 μL) + heated ACM eluate of 10 kDa spin exclusion column (190 μL) 0 100
MRSA (10 μL) + heated ACM eluatee of 5 kDa spin exclusion column (190 μL) 1.34 × 1010 0
MRSA (10 μL) + heated-pronase-treated ACM (190 μL) 0 100
  1. The data are presented as the mean ± SE of three independent experiments. aAn inoculum of ~106 bacterial c.f.u. was used.