Table 1 Acanthamoeba conditioned medium (ACM) exhibited selective antibacterial properties
From: Acanthamoeba and bacteria produce antimicrobials to target their counterpart
| Bacteria + Acanthamoeba conditioned medium (ACM) | CFU remaininga(Bacteria + ACM) | Bacterial kill (%) |
|---|---|---|
| Methicillin-resistant Staphylococcus aureus (MRSA, 10 μL) + ACM (190 μL) | 0 | 100 |
| Acinetobacter (10 μL) + ACM (190 μL) | 1 × 1010 | 0 |
| Pseudomonas aeruginosa (10 μL) + ACM (190 μL) | 6 × 109 | 0 |
| Vancomycin resistant Enterococcus faecalis (VRE, 10 μL) + ACM (190 μL) | 3.75 × 105 | 8.12 ± 9.8 |
| MRSA (10 μL) + heated ACM (190 μL) | 0 | 100 |
| MRSA (10 μL) + heated ACM (180 μL) + PYG (10 μL) | 6.65 × 104 | 93.3 ± 9.9 |
| MRSA (10 μL) + heated ACM (140 μL) + PYG (50 μL) | 1.55 × 107 | 0 |
| MRSA (10 μL) + heated ACM eluate of 10 kDa spin exclusion column (190 μL) | 0 | 100 |
| MRSA (10 μL) + heated ACM eluatee of 5 kDa spin exclusion column (190 μL) | 1.34 × 1010 | 0 |
| MRSA (10 μL) + heated-pronase-treated ACM (190 μL) | 0 | 100 |