Detection of tick-borne ‘ Candidatus Neoehrlichia mikurensis’ and Anaplasma phagocytophilum in Spain in 2013

Table 2 Anaplasmataceae species detected in Ixodes ricinus removed from cows (N = 200) in La Rioja (North of Spain)

Bacterium (no.)	*groESL*				16S rRNA				16S rRNA-EHR
	Ticks, number and stage		Genbank accession no.	Identity (%)	Ticks, number and stage		Genbank accession no.	Identity (%)	Ticks, number and stage		Genbank accession no.	Identity (%)
	Tobía	Jubera	Genbank accession no.	Identity (%)	Tobía	Jubera	Genbank accession no.	Identity (%)	Tobía	Jubera	Genbank accession no.	Identity (%)
‘Ca. N. mikurensis’ (2)	2 M		EU810407	100					2 M		JQ675350	100
A. phagocytophilum (61)
Human pathogenic variant (8)	1 M, 2 F	2 M, 2 F	U72628	99.9-100	1 F*	2 F	U02521	100
Non-pathogenic variants (53)	1 M		AF478558	100	1 M		JN181071	100
	1 M		AF478563	100
	1 F		AY281831	100		1 F	EU839849	100
	2 F	2 M, 7 F	EU246959	99.8-100	1 F	1 M, 4 F	JN181071	100
	5 F	2 M, 29 F	AF548385	99.8-100	2 F	1 M, 11 F^†	JN181071	99.9-100
		1 M	AY281830	100		1 M	JN181071	100

No.: number; ‘Ca. N. mikurensis’: ‘Candidatus Neoehrlichia mikurensis’; A. phagocytophilum: Anaplasma phagocytophilum; M: Male; F: Female; *: only amplified with 16S rRNA fragment gene; ^†: Two of them only amplified with 16S rRNA fragment gene.