Figure 1
Anti- Leishmania reactivity in serum from dogs submitted to different vaccination protocols before and after intradermal challenge with L. infantum plus SGE: C (control;
); Sal (SGE;
); LBSal (killed L. braziliensis vaccine plus SGE;
); LBSapSal (killed L. braziliensis vaccine plus saponin plus SGE;
). IgG2/IgG1 ratio: C (control;
) and LBSapSal (killed L. braziliensis vaccine plus saponin plus SGE;
). (A) anti–L. infantum total IgG; (B) anti–L. infantum IgG1; (C) anti–L. infantum IgG2; (D) IgG2/IgG1 ratio: C (control; white square) and LBSapSal (killed L. braziliensis vaccine plus saponin and SGE; black square), the x-axis displays the times at which the assays were conducted (Tbc: time before challenge with L. infantum; and 20, 90, 274, 435, 541, and 885 days after challenge with L. infantum), and the y-axis represents the mean ELISA absorbance values determined at 492 nm in serum samples diluted 1:80 for IgG total and subclasses. The cut-off is represented by the dotted line. Significant differences (p < 0.05) between the LBSapSal group and the C, Sal, and LBSal groups are indicated, respectively, by the letters a, b, and c. *Significant difference (p < 0.05) between the LBSapSal group and the control C group in IgG2/IgG1 ratio.