Figure 4
Quantification of parasite burden in spleen samples at 885 dac (days after challenge with L. infantum plus SGE) for vaccinated groups: C (control;
); Sal (SGE;
); LBSal (killed L. braziliensis vaccine plus SGE;
); LBSapSal (killed L. braziliensis vaccine plus saponin plus SGE; 
). The y-axis displays the quantification of amastigote forms of Leishmania per milligram of spleen using real-time PCR with specific primers for a single-copy gene of DNA polymerase of Leishmania infantum. The y-axis displays inside the respective square of group the parasitism reduction (%) in Sal, LBSal, and LBSapSal in comparison with the C group. Results were plotted representing median values for each group. Significant differences (p < 0.05) between the LBSapSal and LBSal groups and the C group are represented by the letter a. C = mean 1657; Sal = mean 823, reduction = 50%; LBSal = mean 667, reduction = 60%; LBSapSal = mean 523, reduction = 69%.