Survey of Toxoplasma gondii and Neospora caninum, haemotropic mycoplasmas and other arthropod-borne pathogens in cats from Albania

Table 1 Summary of specific PCR and real-time PCR methods used in this study on cats from Albania

Target	Primers 5′-3′	Cycle conditions	Reference
Conventional PCR methods
Bartonella spp. 16S-23S ITS (154–260 bp) B. henselae: 172 bp	Barhen1_for: YCTTCGTTTCTCTTTCTTCA	44 cycles: 30 Sec 94°C, 30 Sec 60°C, 30 Sec 72°C	[12]
	Barhen2_rev: AACCAACTGAGCTACAAGCC	44 cycles: 30 Sec 94°C, 30 Sec 60°C, 30 Sec 72°C	[12]
Mycoplasma haemofelis/Candidatus M. haemominutum 16S rRNA gene (274 bp/202 bp respectively)	OHOK1_for: ATGCCCCTCTGTGGGGGATAGCCG	35 cycles: 45 Sec 94°C, 45 Sec 58°C, 45 Sec 72°C	[13]
	CaB2_for: CTGGGAAACTAGAGCTTCGCGAGC
	OOCBr1_rev: ATGGTATTGCTCCATCAGACTTTCG
Real-time PCR methods
Leishmania infantum Kinetoplast ~700 bp	Lsh-kF: CTTTTCTGGTCCTCCGGGTAGG	All Real-time PCR methods: 2 min 50°C, 10 min 95°C, 40 cycles: 15 Sec 95°C, 1 min 60°C	[14]
	Lsh-kR: CCACCCGGCCCTATTTTACACCAA
	Lsh-kp: FAM-TTTTCGCAGAACGCCCCTACCCGC-BHQ1
Anaplasma phagocytophilum: Msp2 gene (77 bp)	ApMSP2f: ATGGAAGGTAGTGTTGGTTATGGTATT		[15]
	ApMSP2r: TTGGTCTTGAAGCGCTCGTA
	ApMSP2p-FAM: TGGTGCCAGGGTTGAGCTTGAGATTG
Candidatus M. turicensis 16S rRNA gene (85 bp)	HS Real2_for: GAAGGCCAGACAGGTCGTAAAG		[16]
	HS Real2_rev: CTGGCACATAGTTWGCTGTCACTTA
	HS RealT: FAM-AAATTTGATGGTACCCTCTGA-MGB

ISSN: 1756-3305