The ANTIDotE approach. Identification: Using state of the art proteomic and transcriptomic approaches we will identify novel tick salivary gland proteins. Characterization: These novel tick salivary gland proteins will be characterized using in silico, in vitro and in vivo techniques. Proof of concept: Promising candidates will be assessed in a proof of concept study as anti-tick vaccines to protect against tick-borne diseases. Both transmission as well as tick feeding parameters will be assessed. The symbols (from left to right) represent Babesia, Borrelia nd TBEV, respectively. Valorization plans: Through an integrated and multidisciplinary approach involving European public health institutes, health organizations and industrial companies, we will examine how to develop anti-tick vaccines and implement these in public health systems in Europe.