Mating compatibility in the parasitic protist Trypanosoma brucei

Parasites & Vectors

Table 1 F1 crosses and back crosses

Cross	Total no. of clones	DNA content^a			Hybrids (H)		Selfers^b(S)		Parent (P)		% clones
Cross	Total no. of clones	No.	2 N	>2 N	No.	Genotypes	No.	Genotypes	2 N	>2 N	H	S	P
J10 RFP x 1738 GFP^c	63	29	19	10	63	29	0	0	0	0	100%	0%	0%
F1 crosses
A: F1G1 x F1R1	20	19	4	15	7	7	3	3 (F1G1)	0	5 F1R1, 5 F1G1	35%	15%	50%
B: F1G2 x F1R2	8	5	5	0	3	2	2	1 (F1R2)	3 F1G2	0	38%	25%	38%
C: F1G2 x F1R1	13	13	11	2	11	9	1	1 (F1R1)	1 F1R1	0	85%	8%	8%
D: F1G1 x F1R2	12	12	5	7	1	1	4	3 (F1G1)	3 F1G1	4 F1G1	8%	33%	58%
Back crosses
E: F1G1 x J10 RFP	15	15	10	5	5	3	1	1 (F1G1)	6 F1G1	3 F1G1	33%	7%	60%
F: F1G1 x 1738 RFP	11	11	1	10	8	5	3	2 (F1G1)	0	0	73%	27%	0%
G: F1G2 x J10 RFP	24	23	11	12	12	9	1	1 (F1G2)	6 F1G2	5 F1G2	50%	4%	46%
H: F1G2 x 1738 RFP	10	9	2	7	0	0	6	5 (F1G2)	1 F1G2	3 F1G2	0%	60%	40%
I: F1R1 x J10 GFP	7	7	5	2	2	2	5	2 (F1R1)	0	0	29%	71%	0%
J: F1R1 x 1738 GFP	18	15	12	3	4	1	0	0	12 F1R1	2 F1R1	22%	0%	78%
K: F1R2 x J10 GFP	33	30	27	3	3	2	24	5 (F1R2)	4 F1R2	2 F1R2	9%	73%	18%
L: F1R2 x 1738 GFP	6	4	3	1	6	4	0	0	0	0	100%	0%	0%

^aDNA contents were classified as 2 N (mean 1.02, range 0.78 – 1.17; N = 98) or >2 N (mean 1.68, range 1.21 – 2.14; N = 67) standardized against the DNA content (= 1.00) of a control sample T. b. brucei 427.
^bIncludes clones that have homozygous microsatellite alleles where the parent was heterozygous, clones that are identical to the parent except that they have lost the GFP gene, clones that are identical to the parent except that they have a non-parental karyotype. Parent of origin in brackets.
^cResults taken from [11].

ISSN: 1756-3305