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Table 1 F1 crosses and back crosses

From: Mating compatibility in the parasitic protist Trypanosoma brucei

Cross Total no. of clones DNA contenta Hybrids (H) Selfersb(S) Parent (P) % clones
No. 2 N >2 N No. Genotypes No. Genotypes 2 N >2 N H S P
J10 RFP x 1738 GFPc 63 29 19 10 63 29 0 0 0 0 100% 0% 0%
F1 crosses              
A: F1G1 x F1R1 20 19 4 15 7 7 3 3 (F1G1) 0 5 F1R1, 5 F1G1 35% 15% 50%
B: F1G2 x F1R2 8 5 5 0 3 2 2 1 (F1R2) 3 F1G2 0 38% 25% 38%
C: F1G2 x F1R1 13 13 11 2 11 9 1 1 (F1R1) 1 F1R1 0 85% 8% 8%
D: F1G1 x F1R2 12 12 5 7 1 1 4 3 (F1G1) 3 F1G1 4 F1G1 8% 33% 58%
Back crosses              
E: F1G1 x J10 RFP 15 15 10 5 5 3 1 1 (F1G1) 6 F1G1 3 F1G1 33% 7% 60%
F: F1G1 x 1738 RFP 11 11 1 10 8 5 3 2 (F1G1) 0 0 73% 27% 0%
G: F1G2 x J10 RFP 24 23 11 12 12 9 1 1 (F1G2) 6 F1G2 5 F1G2 50% 4% 46%
H: F1G2 x 1738 RFP 10 9 2 7 0 0 6 5 (F1G2) 1 F1G2 3 F1G2 0% 60% 40%
I: F1R1 x J10 GFP 7 7 5 2 2 2 5 2 (F1R1) 0 0 29% 71% 0%
J: F1R1 x 1738 GFP 18 15 12 3 4 1 0 0 12 F1R1 2 F1R1 22% 0% 78%
K: F1R2 x J10 GFP 33 30 27 3 3 2 24 5 (F1R2) 4 F1R2 2 F1R2 9% 73% 18%
L: F1R2 x 1738 GFP 6 4 3 1 6 4 0 0 0 0 100% 0% 0%
  1. aDNA contents were classified as 2 N (mean 1.02, range 0.78 – 1.17; N = 98) or >2 N (mean 1.68, range 1.21 – 2.14; N = 67) standardized against the DNA content (= 1.00) of a control sample T. b. brucei 427.
  2. bIncludes clones that have homozygous microsatellite alleles where the parent was heterozygous, clones that are identical to the parent except that they have lost the GFP gene, clones that are identical to the parent except that they have a non-parental karyotype. Parent of origin in brackets.
  3. cResults taken from [11].