Figure 1
From: Mapping of the complement C9 binding domain on Trichinella spiralis paramyosin
Binding of native Ts -Pmy to human C9 was identified using immunoprecipitation. The extracts of T. spiralis adult worms were incubated with human C9 and anti-C9 mAb and precipitated with protein G beads. The binding complex was separated by 12% SDS-PAGE and probed with anti-Ts-Pmy mAb 7E2. M, standard protein marker; Lane 1, T. spiralis adult worm extracts only; Lane 2, worm extracts incubated with anti-C9 mAb only as a control; Lane 3, worm extracts incubated with human C9 and anti-C9 mAb.