Diagram of r Ts -Pmy fragments and mapping of the Ts -Pmy binding site to human C9. To identify the binding site of Ts-Pmy to human C9, each fragment (with starting and ending positions) was cloned and expressed as a recombinant protein (A). Each recombinant protein (2 μg) starting from large fragments (B) down to smaller fragments (C and D) were subjected to SDS-PAGE and then probed with anti-HisTag mAb (upper) or bound to human C9 and probed with anti-C9 mAb (lower). IRDye 800CW-labeled goat anti-mouse IgG (50 ng/ml) was used as the secondary antibody. The same amount of BSA (2 μg) was loaded as a non-relevant control. M: standard protein marker.