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Table 1 Regions of the 28S large subunit of ribosomal RNA genes (28S rRNA) and nucleotide patterns used to differentiate Opisthorchis viverrini , Clonorchis sinensis , Haplorchis taichui , H. pumilio , and Stellantchasmus falcatus by the pyrosequencing technique

From: Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens

Types (Accession No.)

Nucleotide positions after the 3′ end of sequencing primer

   
 

3

5

7

9

13

15

16

18

19

21

23

26

27

28

29

32

33

35

36

37

39

41

43

45

O. viverrini (HM004188)

C

G

T

G

T/Ca

Ca

T

T

G

C

T

Ca

Aa

C

A

Aa

Ca

C

C

G

Ca

C

C

G

C. sinensis (JF823989)

C

G

T

G

G

T

T

C

G

C

T

T

G

C

A

Cb

Gb

Tb

C

G

T

C

C

G

H. taichui (HM004187)

Cd

TC

G

Ad

G

T

C

C

A

Cd

Td

T

G

TC

G

-

T

C

A

G

T

C

TC

Gd

H. pumilio (HM004191)

Td

TC

G

Gd

G

T

C

C

A

Td

Cd

T

G

TC

G

-

T

C

A

G

T

C

TC

Ad

S. falcatus (KF241630)

T

G

G

G

G

T

C

T

A

T

C

T

G

C

G

-

T

C

A

Te

T

Te

C

G

  1. aNucleotide for identification of O. viverrini; bNucleotide for identification of C. sinensis; cNucleotide for identification of H. taichui and H. pumilio; dNucleotide positions used for discrimination between H. taichui and H. pumilio; eNucleotide for identification of S. falcatus.