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Table 1 Regions of the 28S large subunit of ribosomal RNA genes (28S rRNA) and nucleotide patterns used to differentiate Opisthorchis viverrini , Clonorchis sinensis , Haplorchis taichui , H. pumilio , and Stellantchasmus falcatus by the pyrosequencing technique

From: Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens

Types (Accession No.) Nucleotide positions after the 3′ end of sequencing primer    
  3 5 7 9 13 15 16 18 19 21 23 26 27 28 29 32 33 35 36 37 39 41 43 45
O. viverrini (HM004188) C G T G T/Ca Ca T T G C T Ca Aa C A Aa Ca C C G Ca C C G
C. sinensis (JF823989) C G T G G T T C G C T T G C A Cb Gb Tb C G T C C G
H. taichui (HM004187) Cd TC G Ad G T C C A Cd Td T G TC G - T C A G T C TC Gd
H. pumilio (HM004191) Td TC G Gd G T C C A Td Cd T G TC G - T C A G T C TC Ad
S. falcatus (KF241630) T G G G G T C T A T C T G C G - T C A Te T Te C G
  1. aNucleotide for identification of O. viverrini; bNucleotide for identification of C. sinensis; cNucleotide for identification of H. taichui and H. pumilio; dNucleotide positions used for discrimination between H. taichui and H. pumilio; eNucleotide for identification of S. falcatus.