In vitro and in vivo parasite density curve. (A) In vitro bioluminescent measurement in the IVIS lumina system. Dm28c-luc trypomastigotes (diamond); and intracellular parasite, after 24 hours (square) and 48 hours (triangle) post infection. Three independent experiments were performed. (B) Parasitemia in mice infected with different T. cruzi populations. Animals were infected with 105 trypomastigotes of Dm28c-WT (open squares, n = 10), or Dm28c-luc (solid squares, n = 15). Two independent experiments were performed.