Follow-up of BALB/c mice infected with 105trypomastigotes of transgenic Dm28c-luc strain. Ex vivo quantification of bioluminescent foci, identified in dissected organs in the plate, after subtracting the background signal (liver) from measurement ROI, identified in the images by the automatic ROI tool. Heart, snout, gonads,rectum, tail, and intestine. 14 dpi (A); 21 dpi (B), 28 dpi (C), and 180dpi (D). Mice 1 (white square), mice 2 (gray square), mice 3 (black square). Percentage of bioluminescent radiance in each organ is related to total bioluminescent radiance found in the whole 24-well plate.