Figure 9
Parasite localization in tissues of infected animals. Ex vivo evaluation of amastigote nests in the rectum and anal region (A-H), heart (I-N) and in the snout (O-P) in BALB/c mice infected with 105 trypomastigotes of Dm28c-luc or Dm28c-WT. Rectum of uninfected mice, control (A). Rectum of mice infected with transgenic Dm28c-luc stain (B-E): 14 dpi (B-C), 21 dpi (D), 28 dpi (E). Rectum of mice infected with Dm28c-WT strain (F-H): 14 dpi (F), 21 dpi (G), 28 dpi (H). Heart of mice infected with transgenic Dm28c-luc stain (I-K and N): 14 dpi (I-J), 21 dpi (K) 28 dpi (N). Heart of mice infected with Dm28c-WT (L-M): 14 dpi (L) and 21 dpi (M). Snout of mice infected with transgenic Dm28c-luc (O) and Dm28c-WT strain (P). Arrows indicate amastigote nests and asterisks inflammatory infiltrate.