Table 2 Performance of four DNA fragments used for species identification of ticks based on their sequences identification success rates using four different methods
Gene | No. of sequences | NN | BLASTn | Liberal tree-based | Liberal tree-based (+threshold) | ||||
|---|---|---|---|---|---|---|---|---|---|
Identified sequences | Rates | Identified sequences | Rates | Identified sequences | Rates | Identified sequences | Rates | ||
COI | 439 | 431 | 98.18% | 430 | 97.95% | 409 | 93.17% | 403 | 91.80% |
16S | 820 | 799 | 97.44% | 786 | 95.85% | 742 | 90.49% | 732 | 89.27% |
ITS2 | 394 | 379 | 96.19% | 381 | 96.70% | 374 | 94.92% | 372 | 94.42% |
12S | 765 | 749 | 97.91% | 751 | 98.17% | 706 | 92.29% | 698 | 91.24% |