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Table 2 Results of nucleotide sequence analysis corresponding to 59 PCR products amplified 1 from the 71 Pulex irritans of this study (44 specimens from Pastaza and 27 from Chimborazo)

From: `Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

Gene Flea gender2 Location Length of sequence (bp) % identity Bacteria with closest identity in the BLAST search Genbank
16S rRNA 9/16 M; 10/28 F Pastaza 1287-1386 98.3-100 Wolbachia sp. wRi endosymbiont of Drosophila simulans NR074437
11/13 M; 13/14 F Chimborazo
4/16 M; 2/28 F Pastaza 1338-1383 98.4-98.7 Wolbachia sp. endosymbiont of Pseudolynchia canariensis DQ115538
0/16 M; 2/28 F Pastaza 1300-1372 98.3-99.3 Wolbachia sp. endosymbiont of Gryllus crickets U83094
0/16 M; 1/28 F Pastaza 1305 99.3 Wolbachia sp. endosymbiont of Gryllus ovisopis U83093
0/16 M; 1/28 F Pastaza 1374 98.3 Wolbachia sp. endosymbiont of Curculio hachijoensis AB746399
0/16 M; 6/28 F Pastaza 1291-1368 98.3-99 Wolbachia sp. endosymbiont of Kleidocerys resedae JQ726770
  1. 1In two cases we did not obtain enough good-quality sequences to identify the bacteria, and we did not obtain amplicons for ten specimens.
  2. 2Number of positive specimens / Total number of specimens from each flea gender; M: Male; F: Female.