Table 2 Results of nucleotide sequence analysis corresponding to 59 PCR products amplified 1 from the 71 Pulex irritans of this study (44 specimens from Pastaza and 27 from Chimborazo)
Gene | Flea gender2 | Location | Length of sequence (bp) | % identity | Bacteria with closest identity in the BLAST search | Genbank |
|---|---|---|---|---|---|---|
16S rRNA | 9/16 M; 10/28 F | Pastaza | 1287-1386 | 98.3-100 | Wolbachia sp. wRi endosymbiont of Drosophila simulans | NR074437 |
11/13 M; 13/14 F | Chimborazo | |||||
4/16 M; 2/28 F | Pastaza | 1338-1383 | 98.4-98.7 | Wolbachia sp. endosymbiont of Pseudolynchia canariensis | DQ115538 | |
0/16 M; 2/28 F | Pastaza | 1300-1372 | 98.3-99.3 | Wolbachia sp. endosymbiont of Gryllus crickets | U83094 | |
0/16 M; 1/28 F | Pastaza | 1305 | 99.3 | Wolbachia sp. endosymbiont of Gryllus ovisopis | U83093 | |
0/16 M; 1/28 F | Pastaza | 1374 | 98.3 | Wolbachia sp. endosymbiont of Curculio hachijoensis | AB746399 | |
0/16 M; 6/28 F | Pastaza | 1291-1368 | 98.3-99 | Wolbachia sp. endosymbiont of Kleidocerys resedae | JQ726770 |