Heligmosomoides neopolygyrus Asakawa & Ohbayashi, 1986, a cryptic Asian nematode infecting the striped field mouse Apodemus agrarius in Central Europe

Table 2 Primer pairs used in the study (F- forward, R- reversed)

Amplified gene	Primers (5➔3’)	Amplified fragment length (bp)	Reference
CO1	F: GGTCAACAAATCATAAAGATATTGG	559	[5]
CO1	R: TAAACTTCAGGGTGACCAAAAAATCA	559	[5]
cytb	F: GRAATTTTGGTAGTATRTTRG	616	[9]
cytb	R: AGMACGYAAAATWGYAWAAGC	616	[9]
ITS1	F: TTGAACCGGGTAAAGTCGT	387 – 423	[5],[13]
ITS1	R: ACAACCCTGAACCAGACGTG	387 – 423	[5],[13]
ITS2	F: ACGTCTGGTTCAGGGTTGT	276 – 306	[5],[14]
ITS2	R: TTAGTTTCTTTTCCTCCGCT	276 – 306	[5],[14]
28S rDNA	F: ACCCGCTGAATTTAAGCAT	619	[15]
28S rDNA	R: TCCGTGTTTCAAGACGG	619	[15]

ISSN: 1756-3305