Comparison of PCR methods for detection of Leishmania siamensis infection

Parasites & Vectors

Table 1 PCR methods and primers used in this study

Target gene	Primer set	Primer sequence 5′-3′	Annealing (°C)	Expected amplicon size (bp)	Ref.
ITS1	L5.8S	TGA TAC CAC TTA TCG CAC TT	53	348	[13]
ITS1	LITSR	CTG GAT CAT TTT CCG ATG	53	348	[13]
*cpb*	LH-CPBEF-F2	TGC GGS TCS TGC TGG GCS TTC	59	525	This study
*cpb*	LH-CPBEF-R	GCG SAY GTA SCC CTT CTC RC	59	525	This study
*cyt b*	L.cyt-S	GGT GTA GGT TTT AGT YTA GG	55	900	[12]
*cyt b*	L.cyt-R	CTA CAA TAA ACA AAT CAT AAT ATR CAA TT	55	900	[12]
*hsp70*	HSP70senKS	GAC GGT GCC KGC STA CTT CAA	61	1422	[15], This study
*hsp70*	HSP70ant	CCG CCC ATG CTC TGG TAC ATC	61	1422	[15], This study
mini-exon	Fme	TAT TGG TAT GCG AAA CTT CCG	54	540	[16]
mini-exon	Rme	ACA GAA ACT GAT ACT TAT ATA GCG	54	540	[16]
SSU-rRNA	S4	GAT CCA GCT GCA GGT TCA CC	67	540	[17]
SSU-rRNA	S12	GGT TGA TTC CGT CAA CGG AC	67	540	[17]
*tim*	TIM-F	TCA ACG AKC ATC AGA TCG AC	53	500	This study
*tim*	TIM-R	ATC TTC TCG CTC ACC CAC TG	53	500	This study

ISSN: 1756-3305