From: Comparison of PCR methods for detection of Leishmania siamensis infection
Target gene | PCR results by | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Number of parasites/μl* | DNA concentration (pg/μl) | |||||||||||
500 | 50 | 5 | 0.5 | 0.05 | 0.005 | 23000 | 2300 | 230 | 23 | 2.3 | 0.23 | |
ITS1 | + | + | + | + | + | - | + | + | + | + | + | - |
cpb | + | + | + | - | - | - | + | + | + | - | - | - |
cyt b | + | + | + | - | - | - | + | + | + | - | - | - |
hsp70 | + | + | + | + | - | - | + | + | + | + | - | - |
mini-exon | + | + | + | + | - | - | + | + | + | + | - | - |
SSU-rRNA | + | + | + | + | + | - | + | + | + | + | + | - |
tim | + | + | + | + | - | - | + | + | + | + | - | - |