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Table 2 The detection limit of seven PCR methods for the detection of L. siamensis

From: Comparison of PCR methods for detection of Leishmania siamensis infection

Target gene

PCR results by

Number of parasites/μl*

DNA concentration (pg/μl)

500

50

5

0.5

0.05

0.005

23000

2300

230

23

2.3

0.23

ITS1

+

+

+

+

+

-

+

+

+

+

+

-

cpb

+

+

+

-

-

-

+

+

+

-

-

-

cyt b

+

+

+

-

-

-

+

+

+

-

-

-

hsp70

+

+

+

+

-

-

+

+

+

+

-

-

mini-exon

+

+

+

+

-

-

+

+

+

+

-

-

SSU-rRNA

+

+

+

+

+

-

+

+

+

+

+

-

tim

+

+

+

+

-

-

+

+

+

+

-

-

  1. *Ten-fold serial dilutions were performed to obtain samples containing 500, 50, 5, 0.5, 0.05 and 0.005 promastigotes/μl before DNA extraction.