Table 4 Sensitivities, specificities and positive and negative predictive values of PCR methods to detect L. siamensis compared with the ITS1-PCR methods
From: Comparison of PCR methods for detection of Leishmania siamensis infection
Method | Result | L. siamensis | Sensitivity % | Specificity % | PPV % | NPV % | |
|---|---|---|---|---|---|---|---|
+ve | -ve | ||||||
no. (%) | no. (%) | (95% CI) | (95% CI) | (95% CI) | (95% CI) | ||
cpb | +ve | 8 (100.0) | 16 (47.1) | 100 (63.1-100.0) | 52.9 (35.1-70.2) | 33.3 (15.6-55.3) | 100 (81.5-100.0) |
-ve | 0 (0) | 18 (52.9) | |||||
cyt b | +ve | 3 (37.5) | 0 (0) | 37.5 (8.52-75.5) | 100 (89.7-100.0) | 100 (29.2-100.0) | 87.2 (72.6-95.7) |
-ve | 5 (62.5) | 34 (100.0) | |||||
hsp70 | +ve | 7 (87.5) | 7 (22.8) | 87.5 (47.3-99.7) | 79.4 (62.1-91.3) | 50 (23.0-77.0) | 96.4 (81.7-99.9) |
-ve | 1 (12.5) | 27 (77.2) | |||||
mini-exon | +ve | 7 (87.5) | 0 (0) | 87.5 (47.3-99.7) | 100 (89.7-100.0) | 100 (59.0-100.0) | 97.1 (85.1-99.9) |
-ve | 1 (12.5) | 34 (100.0) | |||||
SSU-rRNA | +ve | 8 (100.0) | 6 (17.6) | 100 (63.1-100.0) | 82.4 (65.5-93.2) | 57.1 (28.9-82.3) | 100 (87.7-100.0) |
-ve | 0 (0) | 28 (82.4) | |||||
tim | +ve | 4 (50) | 0 (0) | 50 (15.7-84.3) | 100 (90.7-100.0) | 100 (39.8-100.0) | 90.5 (77.4-97.3) |
-ve | 4 (50) | 38 (100.0) | |||||