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Figure 1 | Parasites & Vectors

Figure 1

From: Application of qPCR in conjunctival swab samples for the evaluation of canine leishmaniasis in borderline cases or disease relapse and correlation with clinical parameters

Figure 1

qPCR1 sensitivity without (A) or with (B) raw CS lysate. Serial dilutions of MHOM/TN/80/IPT1 L. infantum DNA (ranging from 1 to 0.001 parasite equivalent) were amplified in triplicate (1 μl in a total of 25 μl reaction mixture) (panel A). To evaluate the eventual inhibitory effects, 1 μl of raw lysate was spiked in the PCR tubes containing the dilutions of MHOM/TN/80/IPT1 L. infantum DNA described above (panel B).

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Parasites & Vectors

ISSN: 1756-3305

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