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Table 2 Leishmania parasite quantification in buffy coat (BC) and conjunctival swab (CS) samples, by qPCR1 (normal text) and by qPCR2 (bold text)

From: Application of qPCR in conjunctival swab samples for the evaluation of canine leishmaniasis in borderline cases or disease relapse and correlation with clinical parameters

Group IFAT titre Dog ID BC qPCR (par/ml) Left CS qPCR (par/5×104cells) Right CS qPCR (par/5×104cells)
A < 1:40 1 0.01 0.00 0.00
n.a. n.a. n.a.
2, 5 0.00 n.a. n.a.
n.a. n.a. n.a.
4 22.27 97.84 9.65
10.40 23.16 0.00
6 0.04 n.a. n.a.
n.a. n.a. n.a.
7 0.03 0.00 0.00
n.a. n.a. n.a.
12 4.26 0.00 0.00
6.45 n.a. n.a.
3, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 0.00 0.00 0.00
n.a. n.a. n.a.
B 1:40 – 1:80 25, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 41 0.00 0.00 0.00
n.a. n.a. n.a.
26 0.07 0.00 0.00
n.a. n.a. n.a.
27 n.a. 0.05 0.00
n.a. 0.15 0.00
39 0.00 0.00 n.a.
n.a. n.a. n.a.
40 0.00 0.01 0.06
n.a. 0.06 0.89
C ≥ 1:160 42 0.00 0.08 0.00
n.a. n.a. n.a.
43 0.7 n.a. n.a.
n.a. n.a. n.a.
44 1.16 0.00 0.00
0.00 n.a. n.a.
45 0.10 86.50 3.55
0.14 688.97 36.35
46 0.43 0.00 0.00
4.45 n.a. n.a.
47 0.00 0.00 0.02
n.a. n.a. n.a.
48 0.21 0.00 0.00
0.00 n.a. n.a.
49, 51, 52, 53, 54, 55, 56 0.00 0.00 0.00
n.a. n.a. n.a.
50 0.07 0.00 0.00
n.a. 0.00 0.00
57 0.00 0.00 1.83
n.a. n.a 3.66
D ≥ 1:160 58 0.52 0.00 0.00
3.40 0.00 0.00
59 1.49 n.a. n.a.
5.00 n.a. n.a.
60 2.83 2.37 3.97
19.20 7.43 15.90
61 1.43 999.28 184.77
1.28 1625.05 372.43
62 8.00 0.46 8.00
0.77 n.a. 63.90
63 0.00 0.98 0.70
n.a. 3.01 n.a.
64 0.00 0.00 2.10
n.a. n.a. 28.82
65 1.72 1659.79 808.35
11.93 4690.98 3147.88
66 0.00 88.03 33.44
0.00 29.87 71.67
67 0.35 9.36 9.80
0.27 4.25 3.67
68 0.00 4.11 70.96
n.a. 7.59 57.75
69 0.00 189.23 16.42
n.a. 129.50 30.96
70 0.12 3.21 n.a.
0.18 8.38 n.a.
71 0.00 32.60 19.38
n.a. 77.64 39.74
72 0.00 1.13 49.54
0.00 6.72 227.29
73 0.88 33.35 184.18
4.94 139.98 683.95
74 0.56 13.08 11.73
4.68 24.74 9.50
75 264.28 85.94 183.85
1315.97 717.96 1969.04
76 0.34 5.89 12.22
1.52 50.43 56.03
77 2.96 44.31 50.67
10.87 278.25 282.91
78 n.a. 0.00 0.00
n.a. n.a. n.a.
79 19.38 616.82 74.82
n.a. 2609.35 222.93
80 0.00 0.26 0.44
n.a. 0.85 0.35