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Figure 2 | Parasites & Vectors

Figure 2

From: Wolbachia lipoproteins: abundance, localisation and serology of Wolbachia peptidoglycan associated lipoprotein and the Type IV Secretion System component, VirB6 from Brugia malayi and Aedes albopictus

Figure 2

Localisation of PAL on Wolbachia membranes. A, B – Western immunoblot detection of wPAL protein in B. malayi (A) and C6/36 mosquito cell line fractional (B) samples. A. Protein samples of B. malayi infected w Bm (1) and B. malayi after six weeks tetracycline treatment (2). B. Protein samples of infected and non-infected C6/36 cells cultivated in vitro: 1 – total protein extract of non-infected cells, 2 – membrane fraction of non-infected cells, 3 – total protein extract of cells infected with w AlbB, 4 – membrane fraction of infected cells, 5, 6 – cytoplasmic fractions of non-infected and infected cells, respectively. M – marker, kDa. C-F – Immuno-transmission electron microphotographs showed localisation of PAL on the bacterial membrane in cytoplasm of adult B. malayi infected with wBm. b – bacteria. Arrow head indicates membrane of vacuole containing Wolbachia.

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Parasites & Vectors

ISSN: 1756-3305

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