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Figure 4 | Parasites & Vectors

Figure 4

From: Wolbachia lipoproteins: abundance, localisation and serology of Wolbachia peptidoglycan associated lipoprotein and the Type IV Secretion System component, VirB6 from Brugia malayi and Aedes albopictus

Figure 4

Visualisation of PAL and VirB6 in whole worm mounts of B. malayi . A, B, Wolbachia (small red foci, bacteria DNA stained by propidium iodide, large red spot, nematode nuclei, see Methods) and PAL (green, secondary antibody labeled by FITC) in lateral cord of filarial nematode, C, merged A and B. D, E, VirB6 (green) and Wolbachia (small red foci) in B. malayi (wBm). F, merged D and E. G, H, negative control – Wolbachia-free B. malayi treated with antibiotic (lack of PAL reactivity shown as representation of both antibodies). I, merged G and H.

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