Figure 1

TcNTPDase-1 expression in epimastigote forms from distinct T. cruzi isolates. A. TcNTPDase-1 mRNA levels estimated by RT-qPCR. The relative quantification by ΔΔCt method was performed using the avirulent Cl-14 clone as calibrator. B. Ecto-ATPase and ecto-ADPase activities between distinct T. cruzi strain/clones. The ecto-nucleotidase activities were estimated in T. cruzi epimastigotes, using 5 mM ATP or ADP as substrate. The inset shows the ecto-nucleotidase activities represented as Fold Change versus Cl-14 clone. C. Alignment of DNA sequence from PCR products for the TcNTPDase-1. The asterisks indicate identity between the nucleotides. *, # p < 0.05 (versus Cl-14 clone, Mann-Whitney Rank-Sum test).