Figure 1

Nucleotide and translated open reading frame (ORF) sequences for RmAQP1 . Nucleotide sequence numbering starts from the 5′ end of the transcript as determined by RACE. Amino acid numbering begins from the start methionine amino acid. The entire ORF is in bold text, while the portion of the ORF used as the vaccine antigen is underlined. The six predicted transmembrane helices (aa # 11-31, 43-63, 88-108, 144-164, 176-196, and 231-251) and the two NPA motifs (aa # 68-70 and 201-203) are highlighted. The predicted phosphorylation sites at 8 serine (aa # 64, 77, 207, 265, 280, 287, 295, and 297), 3 threonine (aa # 107, 123, and 223), and 1 tyrosine (aa # 229) amino acid residues are indicated in outline font. Multiple in-frame stop codons are present both upstream of the presumptive methionine start and downstream of the stop TAG codon.