Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis

Table 1 Effect of vaccination using recombinant HlFERs on tick feeding and reproduction parameters

Experimental group	Engorged weight (mg)	R_W^a(%)	Egg weight (mg)	R_O^a(%)	Ticks with hatched eggs (%)	R_H^a(%)	Ticks with dead larvae (%)	E^b(%)
Adjuvant (control)	261.2 ± 51.9	0	170.0 ± 40.4	0	100.0	0	0	--
rHlFER1	243.4 ± 77.3	6.8	142.8 ± 62.5*	12.4	82.0*	18.0	5.5	34.0
rHlFER2	218.0 ± 66.0*	16.5	126.5 ± 55.8*	22.4	80.0*	20.0	5.7	49.0

Data represent average values from ticks infested on three rabbits for each group, from two separate vaccination trials.
^aFormulas for the calculation of reductions in engorged weight (R_W), oviposition (R_O), and hatch (R_H) are described in the Methods section.
^bVaccine efficacy (E) was calculated by comparing tick engorged weight, tick egg weight, and the number of ticks with completely hatched eggs from the recombinant HlFER1 (rHlFER1) or recombinant HlFER2 (rHlFER2) group with those from the control group. The formula is described in the Methods section.
*Significantly different vs. control (P < 0.05, Student’s t- test).

ISSN: 1756-3305