Figure 3
From: Plasmodium falciparum, but not P. vivax, can induce erythrocytic apoptosis
Cell membrane integrity and cell volume in erythrocytic apoptosis induced by plasma. RBC from a healthy individual were incubated for 48 h in the presence of plasma from non-infected control healthy individuals or malaria patients. (A-C) Representative flow cytometry analysis of cell membrane integrity accessed by calcein-AM and annexin V-APC double staining in RBC incubated with plasma from non-infected individuals (A) or from P. vivax (B) or P. falciparum (C) patients. (D) Forward scatter of RBC exposing (AnV+) or not (AnV-) phosphatidylserine after incubation with plasma from control non-infected individuals or patients infected by P. vivax (Pv) or P. falciparum (Pf). Statistical difference in (D) was tested by non-parametric Mann Whitney test.