Figure 8From: miR-281, an abundant midgut-specific miRNA of the vector mosquito Aedes albopictus enhances dengue virus replicationDepletion of miR-281 drastically affects DENV-2 abundance in female mosquitoes. Knock-down of miR-281 expression by miR-281 antagomiRs significantly down-regulates DENV-2 gRNA. DENV-2 gRNA was quantified by RT-qPCR and normalized to the rpS7 gene. The RT-qPCR data were analyzed using the 2-Δct method. Data shown represent three independent experiments with three triplicates each. P values were calculated using ANOVA.Back to article page