Figure 5

Expression patterns of Hc-age-1 and Hc-aap-1 . Representative expression patterns of Ce-age-1, Hc-age-1, Ce-aap-1 and Hc-aap-1 displayed in Caenorhabditis elegans transformed by either of the four green fluorescent protein (GFP) constructs pL-Cagep, pL-Hagep, pL-Caapp and pL-Haapp (Additional file 2). Three transgenic lines were produced for each of the four plasmids. (a and b) Differential interference contrast (DIC) (a) and fluorescence (b) images of transgenic third-stage larva (L3) of C. elegans expressing Ce-age-1 p::gfp (pL-Cagep). (c and d) DIC (c) and fluorescence (d) images showing the expression pattern of Hc-age-1 p::gfp (pL-Hagep) in first-stage larva (L1). (e and f) DIC (e) and fluorescence (f) images, showing the expression pattern of Hc-age-1 p::gfp (pL-Hagep) in L3. (g and h) DIC (g) and fluorescence (h) images showing expression pattern of Ce-aap-1 p::gfp (pL-Caapp) in L3. (I and J) DIC (i) and fluorescence (j) images showing expression pattern of Hc-aap-1 p::gfp (pL-Haapp) in L3. Scale bars =50 μm.