Figure 2From: Characterisation of the ex vivo virulence of Leishmania infantum isolates from Phlebotomus perniciosus from an outbreak of human leishmaniosis in Madrid, SpainExamination of infection of BMMØ and BMDC at 24 h after infection. Infected cultures were washed to remove free parasites, fixed in methanol, stained with Giemsa and examined under a light microscope. Intracellular amastigotes (arrows) were clearly visible.Back to article page