Neighbour-joining (NJ) phylogram reconstructed using the newly-generated and retrieved from GenBank ITS1-5.8S-ITS2 sequences for Diplostomum spp. Nodal support from Maximum Likelihood (ML) and Bayesian Inference (BI) analyses indicated as NJ/ML/BI. Outgroup: Tylodelphys clavata. The scale-bar indicates the expected number of substitutions per site. Isolates from Spain are coded as in Table 3; stars indicate adult isolates from gulls.