Anti-Mayaro virus activity of Cassia australis extracts (Fabaceae, Leguminosae)

Parasites & Vectors

Table 1 Proposed compounds from ESI(−)FT-ICR MS analyzes

Suggested compounds	Class of natural product	EtOAc fraction	*n-BuOH*fraction	EtOAc-Pp	*m/z* _theoretical	*m/z* _measured	Molecular formula (M)	Error (ppm)	DBE
Kaempferol	Flavonol	+	-	+	285.04046	285.04042	C₁₅H₁₀O₆	0.41	11
Quercetin	Flavonol	+	-	+	301.03538	301.03538	C₁₅H₁₀O₇	0.25	11
Rhamnetin/isorhamnetin	Flavonol	+		+	315.05103	315.05098	C₁₆H₁₂O₇	0.33	11
Quercetin pentoside	Flavonol Monoglycoside	+	+	+	447.09329	447.10361	C₂₁H₂₀O₁₁	0.38	12
Quercetin hexoside	Flavonol Monoglycoside	+	+	+	463.08820	463.08801	C₂₁H₂₀O₁₂	0.38	12
Tricetin-4′-methoxy-3′-â-D-glucoside¹	Flavone Monoglycoside	+	+	+	477.10385	477.10361	C₂₂H₂₂O₁₂	−0.12	12
-	Flavan-3-ol dimer	+	-	+	529.15041	529.15024	C₃₀H₂₆O₉	0.51	18
-	Flavan-3-ol dimer	+	-	+	545.14532	545.14519	C₃₀H₂₆O₁₀		18
-	Flavan-3-ol dimer	+	-	-	561.14024	561.08601	C₃₀H₂₆O₁₁	0.45	18
	Flavan-3-ol dimer	+	-	-	591.11441	591.09653	C₃₀H₂₄O₁₃	0.40	19
Vicenin-2 kaempferol diglycoside¹	Flavone diglycoside	-	+	+	593.15120	593.15104	C₂₇H₃₀O₁₅	−0.08	13
Quercetin dihexoside	Flavonol diglycoside	-	+	+	609.14611	609.14591	C₂₇H₃₀O₁₆	0.06	13
-	Flavan-3-ol trimer	-	-	+	769.22905	769.22868	C₄₅H₃₈O₁₂	0.08	27
	Flavan-3-ol trimer	-	-	+	785.22397	785.22349	C₄₅H₃₈O₁₃	0.08	27

¹Purified compounds identified by NMR, DBE - double bond equivalent, + detected, − not detected, Mass error (ppm) = [(m/z_measured – m/z_theoretical)/m/z_theoretical]*10⁶.

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