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Table 1 The number of A. hoyamushi in tunics in various KS phases, as measured using qPCR

From: Quantitative assessment of Azumiobodo hoyamushi distribution in the tunic of soft tunic syndrome–affected ascidian Halocynthia roretzi using real-time polymerase chain reaction

Unit

Tunic

Kitamura's scale

1

2

3

4

# of A. hoyamushi/ 0.25cm2

BS

2.9 ± 0.4

160.1 ± 16.7

2,994.6 ± 1,783.5

16,066.9 ± 2,609.9

AS

2.9 ± 0.6

46.6 ± 30.3

2,337.6 ± 424.1

4,121.0 ± 281.7

A

0.2 ± 0.2

0.4 ± 0.5

1,912.9 ± 650.3

5,053.2 ± 2,560.8

B

0.0 ± 0.0

38.0 ± 1.1

2,339.4 ± 257.5

5,599.2 ± 915.1

C

0.2 ± 0.0

3.6 ± 0.0

1,362.0 ± 462.1

5,396.1 ± 507.3

# of A. hoyamushi/ GT

BS

106.0 ± 1.3

7,939.6 ± 1,241.9

39,093.3 ± 18,334.2

617,004.1 ± 10,949.2

AS

85.5 ± 1.8

3,012.5 ± 2,607.6

70,866.4 ± 6,961.5

131,835.5 ± 37,352.4

A

6.0 ± 2.6

12.4 ± 13.7

49,108.0 ± 6,987.4

169,404.2 ± 94,002.7

B

0.3 ± 0.4

1,005. 2 ± 44.8

59,569.7 ± 6,555.5

205,291.7 ± 24,037.0

C

3.1 ± 0.6

114.9 ± 23.6

31,702.4 ± 7,770.8

211,555.4 ± 86,528.5

  1. Kitamura's scale (KS)-1: ascidians with no soft tunic syndrome (STS) symptoms but collected from STS-affected population; KS-2: ascidians exhibiting mild STS; KS-3: ascidians with advanced STS; KS-4: seriously diseased ascidians with ruptured tunics.
  2. BS: branchial siphon; AS: atrial siphon.