Construction of MIC2 bait plasmids. (A) Schematic illustration of full-length MIC2, and its different domains used as baits in the yeast-two-hybrid screen. (B) Agarose gel electrophoresis analysis of different fragments of MIC2 amplified from T. gondii cDNA. M: DNA maker; 1: MIC2 ectodomain (aa74 - 596) that lacks propeptide and the sixth TSR motif; 2: A/I domain of MIC2 (aa74 - 270); 3: the TSR domain containing five thrombospondin typeIrepeats of MIC2 (aa271 - 596). (C) Agarose gel electrophoresis analysis of constructed bait plasmids digested with NdeI and SalI. M: DNA maker; 1: pGBKT7-MIC2; 2: pGBKT7-A/I; 3: pGBKT7-TSR; 4: pGBKT7 vector.