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Figure 3 | Parasites & Vectors

Figure 3

From: Identification of host proteins interacting with the integrin-like A domain of Toxoplasma gondii micronemal protein MIC2 by yeast-two-hybrid screening

Figure 3

Analysis of putatively positive hits obtained from the yeast-two hybrid screen. (A) Agarose gel electrophoresis analysis of PCR products obtained from amplification of the inserts on putatively positive prey plasmids. M: DNA maker; Lane 1–8: PCR amplification products of the inserts on the eight putatively positive hits. (B) Confirmation of putative hits. Y2HGold cells were co-transformed with pGBKT7-A/I and each of the eight putatively positive prey plasmids (number 1 to 8) and plated on QDO/X/A plates; positive interaction was indicated by the presence of blue colonies. Co-transformation with pGADT7-T and pGBKT7-Lam was used as a negative control, whereas co-transformation with pGADT7-T and pGBKT7-53 was used as a positive control.

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