Identification of host proteins interacting with the integrin-like A domain of Toxoplasma gondii micronemal protein MIC2 by yeast-two-hybrid screening

Table 1 Primers used to amplify different domains of MIC2 from cDNA of T. gondii tachyzoites

Primer name	Sequence¹	Restriction enzyme sites
pGBKT7-MIC2-F	GC CATATG CAGCTGGACATTTGCTTTC	NdeI
pGBKT7-MIC2-R	TA GTCGAC GACAAGCAGGATACGAACATG	SalI
pGBKT7-A/I-R	TA GTCGAC TGGCATCCTGGGGGAGTGTCTT	SalI
pGBKT7-TSR-F	GC CATATG GCCATTTGCTCGGATTGGTC	NdeI

¹Restriction enzyme sites were presented in italics. Primers were used in the following combinations for PCR amplifications: pGKBT7-MIC2-F and pGKBT7-MIC2-R were used to amplify MIC2 ectodomain (aa74 - 596); pGBKT7-MIC2-F and pGKBT7-A/I-R were used to amplify the A/I domain (aa74 - 270); and pGKBT7-TSR-F and pGKBT7-MIC2-R were used to amplify the TSR domain (aa271 - 596).

ISSN: 1756-3305