Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling

Parasites & Vectors

Table 1 Culicidae used to establish the reference database of MALDI-TOF spectra and arthropods used in the blind test

Species	Geographical origin	Source*	No. of specimens used to create the database	Developmental stage^a	No. of specimens used for the blind test procedure	Log score-values^b[low-High]	Stages identified
An. gambiae(S form)	Montpellier, France (IRD)	UR	6	L2	11	[2.122 - 2.648]	L2, L3
			6	L3	15	[2.173 - 2.548]	L2, L3, L4
			6	L4	16 (2^c)	[2.221 - 2.433]	L3, L4
			5	P1	12	[2.024 - 2.379]	P1
An. coluzzi	Dakar, Sengal	UR	6	L2	6	[2.196 - 2.466]	L2
			6	L3	5	[2.565 - 2.632]	L3
			6	L4	5	[2.325 - 2.499]	L3, L4
			6	P1	9	[2.177 - 2.588]	P1
Ae. albopictus	Montpellier, France (EID)	UR	6	L2	5	[2.023 - 2.435]	L2
			6	L3	12	[1.946 - 2.795]	L2, L3
			6	L4	14	[2.075 - 2.655]	L4, P1
			6	P1	5	[2.121 - 2.288]	P1
Ae. albopictus	Manaus, Brazil	PI	0	L3	5	[2.044 – 2.145]	L3
			0	L4	2	[1.987 - 1.995]	L4, P1
			0	P1	4	[1.940 - 2.239]	P1
Ae. aegypti PAEA	Papeete, Tahiti	PI	2	L2	1	[2.284]	L2
			2	L3	2	[2.241 - 2.468]	L2, L3
			2	L4	3	[1.958 - 2.383]	L4, P1
			2	P1	2	[2.394 - 2.441]	P1
Cx. p. pipiens		PI	2	L2
			2	L3	1	[2.454]	L3
			2	L4	4	[2.143 - 2.419]	L3, L4
			2	P1	3	[2.086 - 2.516]	P1
Cx. p. molestus		PI	2	L2
			2	L3	2	[2.049 - 2.11]	L2, L3
			2	L4	3	[2.504 - 2.03]	L3, L4
			2	P1	1	[2.528]	P1
Total			95		149

*Location of laboratory-reared mosquitoes, UR, URMITE; PI, Pasteur Institute. ^aDevelopmental stages are abbreviated as follows: L, larvae; P1, pupae collected at day 1. ^bRange of identification log score values. ^CNumber of specimens misidentified at the species level.

ISSN: 1756-3305