Table 1 The VNTR loci used in the study and the observed (or predicted for the genome) amplicon sizes from the initial screen for variability
Locus | Repeat length | Repeat motif | Primers (5' to 3') | Observed size (bp) | ||
|---|---|---|---|---|---|---|
Genome | MA | WI | ||||
BMV1 | 6 | AGTTCT or AGGTCA | F- CAATCTATGAGGCATGCGATTC | 346 | 340 | 340 |
R- CTAAAAGGCCCGATGGTTCA | ||||||
BMV2 | 6 | TATAAC | F-TCCAGTGACAATGACATATTTAAGCA | 400 | 405 | 392 |
R- TGTCCTCATTCTGAGCCACAGT | ||||||
BMV4 | 11 | TTAGCTATGGG | F- ACCACCACCAGGCCTCTATG | 405 | 361 | 295 |
R- CTGGACCATGATTTGGTTGA | ||||||
BMV5 | 9 | GCTGTATTT | F- AGGCCCCTGTTCATCACATG | 415 | 317 | 317 |
R- GGAATAGCCTCGAGTCCAGA | ||||||
BMV8 | 10 | ACATACAGCG | F- AGGCCAGTGGAGCAGAGAAG | 262 | 327 | 295 |
R- CAAGCAATCGTCGCTGTATG | ||||||
BMV10 | 3 | GAT | F- TTGTTGGTGTCCGGGTTGTA | 303 | 305 | 298 |
R- ATGCTATTGCCTCGCAACCT | ||||||
BMV13 | 15 | TCCTTACTAGCCTTA | F- ACCGCTCCCGCACTTTAGTA | 351 | 520 | 443 |
R- CCTGCGGGTTCTACCACTCT | ||||||
BMV20 | 6 | ATACTA | F- CAGGGTTTATGCGAAGAGTGG | 713 | 713 | 754 |
R- GTGCTGCAGGCTTCGATGTA | ||||||
BMV23 | 5 | ATATA | F- CCGCCTCTCCTATTCCCCTA | 322 | 275 | 270 |
R- GAAGAACAGTTGGATGACTTCG | ||||||