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Figure 4 | Parasites & Vectors

Figure 4

From: Exploring features and function of Ss-riok-3, an enigmatic kinase gene from Strongyloides stercoralis

Figure 4

Diagram of upstream gene and intergenic regions of the genes Ss-riok-3 ( Strongyloides stercoralis ) and Ce-riok-3 ( Caenorhabditis elegans ), and alignment of promoter elements predicted from the upstream genomic DNA sequences of these two genes. (A) The upstream gene and intergenic regions. Black boxes indicate partial coding sequence, with gene name indicated; a white box indicates an untranslated region (UTR); a line indicates an intergenic region. Termination and initiation codons are marked on the coding strand, and UTRs and intergenic regions marked below white boxes and the lines, respectively. Brackets indicate genomic DNA inserted into the transformation constructs. (B) Alignment of the predicted promoters of Ss-riok-3 and Ce-riok-3. Genomic DNA sequences upstream of the initiation codon of Ss-riok-3 and Ce-riok-3 were aligned. Colored boxes represent the promoter elements: CAAT (CCAAT) or inverse CAAT (ATTGG) motif (blue), inverse GATA (TTATC) (green), GATA (WGATAR) (turquoise), E- (CANNTG) (grey) and TATA (red) boxes. The numbers represent the positions of the nucleotides upstream of the start codon. The thick black line above the Ss-riok-3 promoter indicates the 84 bp 3’-end of the coding sequence of Ss-rep-1. Left arrows above Ss-riok-3 and below Ce-riok-3 indicate the 3’-UTRs of the Ss-rep-1 and Ce-zk632.4. Vertical arrows indicate the transcription start site.

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