Figure 4

Traffic of light vesicles. A, Cytoplasmic connection (CC) between anterior esophagus cell bodies and basal corrugations, showing light vesicles (LV) in transit; debris (D) is evident between the corrugations presumably derived from released content of light vesicles (BI, basal invagination; M, muscle layer; MT, mitochondria). B, Transverse section of cytoplasmic connection reveals microtubules (MI) potentially involved in vesicle transportation (DB, discoid body). C, Anterior esophageal lining showing clusters of light vesicles distributed all the way down the tips of the corrugations. D, Light vesicles near the base of corrugations apparently emptying their cargo to the exterior; mitochondria are also evident whilst the surface is coated by membranous material (arrowed). Scale bar: A 5 μm; B 500 nm; C 2 μm; D 200 nm; E 500 nm; F 200 nm.