Figure 7

Host antibodies bind to antigens in the esophageal lumen. Cryosections were prepared from 24 week adult male worms from mice. A, Test, a longitudinal section showing localization of intrinsic mouse IgG (green), counterstained to highlight the musculature (orange) and nuclei (blue). Both anterior and posterior esophageal compartments are positive but with different patterns of reactivity; the staining of the anterior lining is focal and punctate whilst that of the posterior is mainly along the luminal edge of the plates. B, Control, reacted with AF488-labelled goat anti-hamster antibody; both anterior and posterior compartments are negative. C, High magnification image of anterior esophageal lining showing IgG distributed in intense focal blobs, numerous finer punctate spots and apparent striations (arrowed) that correspond to the individual corrugations. Most blobs are in the 2–3 μm range but one large sphere (6 μm, starred, host cell?) is an exception. Scale bar: A 50 μm; B 50 μm; C 20 μm.