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Table 3 Validation of the MC-PCR on faecal samples from 177 foxes from Switzerland, using the SCT as gold standard

From: A semi-automated magnetic capture probe based DNA extraction and real-time PCR method applied in the Swedish surveillance of Echinococcus multilocularis in red fox (Vulpes vulpes) faecal samples

SCT (worm burden)

Number of samples

MC-PCR positive

Cq-valuesaMean (range)

0

84

18

33.4 (31.4-36.2)

1 - 10

26

21

33.0 (30.7-35.1)

11 - 100

21

17

31.1 (28.8-33.5)

101 - 1000

25

25

29.5 (27.5-29.4)

1001 - 10 000

15

15

27.9 (25.0-29.4)

10 001–100 000

3

3

26.5 (22.1-29.4)

>100 000b

2

0

 

>100 000bc

1

1

27.9

Total samples

177

100

 
  1. aOnly SCT-positive tests where Cq-values were available were included.
  2. bLarge amounts of immature worms, impossible to count.
  3. cLarge amounts of only proglottids, impossible to count.