Role of enhanced vector transmission of a new West Nile virus strain in an outbreak of equine disease in Australia in 2011

Table 1 Infection, dissemination and transmission rates in Culex annulirostris exposed to 10 ^8.1 and 10 ^7.6 TCID ₅₀ /mL of the WNV _KUN2011 and WNV _KUN2009 strains, respectively and tested at different time points post-exposure

	Infection^a				Dissemination^b				Dissemination/infection^c				Transmission^d				Transmission/dissemination^e
Day post exposure	KUN2011		KUN2009		KUN2011		KUN2009		KUN2011		KUN2009		KUN2011		KUN2009		KUN2011		KUN2009
3	76	(19/25)	64	(16/25)	28	(7/25)	32	(8/25)	37	(7/19)	50	(8/16)	0	(0/25)	0	(0/25)	0	(0/7)	0	(0/8)
5	68	(17/25)	68	(17/25)	60	(15/25)	64	(16/25)	88	(15/17)	94	(16/17)	12	(3/25)	12	(3/25)	20	(3/15)	19	(3/16)
7	64	(16/25)	68	(17/25)	64	(16/25)	52	(13/25)	100	(16/16)	76	(13/17)	28	(7/25)	12	(3/25)	44	(7/16)	23	(3/13)
10	64	(16/25)	44	(11/25)	64	(16/25)	44	(11/25)	100	(16/16)	100	(11/11)	44	(11/25)	40	(10/25)	69	(11/16)	91	(10/11)
12	68	(17/25)	60	(9/15)	68	(17/25)	60	(9/15)	100	(17/17)	100	(9/9)	68	(17/25)	53	(8/15)	100	(17/17)	89	(8/9)
14	52	(13/25)	84	(21/25)*	52	(13/25)	76	(19/25)	100	(13/13)	90	(19/21)	52	(13/25)	72	(18/25)	100	(13/13)	95	(18/19)

^aPercentage of mosquitoes containing virus in their bodies (number positive/number tested).
^bPercentage of mosquitoes containing virus in their legs and wings (number positive/number tested).
^cPercentage of infected mosquitoes containing virus in their legs and wings (number positive/number infected).
^dPercentage of mosquitoes containing virus in the saliva expectorates (number positive/number tested).
^ePercentage of mosquitoes with a disseminated infection containing virus in the saliva expectorates (number positive/number disseminated).
*Fisher’s exact test P-value <0.05 for day 14 infection rate.

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