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Table 1 Stability of eRFP cassette in C7/10 cells after five serial passages

From: Eilat virus displays a narrow mosquito vector range

 

Titer

 

EILV-eRFP

(log10PFU/ml)

% of plaques expressing eRFP

(+/− SD)

Passage #1

6.1 (+/− 0.18)

99

Passage #5

6.5 (+/− 0.20)

90

  1. Virus titers for passage one and five were generated with standard plaque assay. Percent of plaques expressing eRFP was determined by counting plaques expressing eRFP via fluorescent microscope and crystal violet staining.
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Parasites & Vectors

ISSN: 1756-3305

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