Figure 1From: The overexpression of genes of thiol metabolism contribute to drug resistance in clinical isolates of visceral leishmaniasis (kala azar) in IndiaComparison of Rhodamine and Calcein accumulation with retention in L. donovani clinical isolates. (A) Rhodamine accumulation in promastigotes of sensitive (2001) and resistant (39, 41, R-5) isolates under normal condition after 45Â minutes of incubation. (B) Rhodamine accumulation in promastigotes of sensitive (2001) and resistant (39, 41, R-5) isolates under ATP depletion after 15Â minutes of incubation. (C) Calcein accumulation in promastigotes of sensitive (2001) and resistant (39, 41, R-5) isolates under normal condition after 60Â minutes of incubation. (D) Calcein accumulation in promastigotes of sensitive (2001) and resistant (39, 41, R-5) isolates under ATP depletion after 30Â minutes of incubation. (E) Calcein retention in promastigotes of sensitive (2001) and resistant (39, 41, R-5) isolates without probenecid. (F) Calcein retention in promastigotes of sensitive (2001) and resistant (39, 41, R-5) isolates with probenecid. Data shown are results from 1 experiment and representative of 3 independent experiments.Back to article page