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Figure 4 | Parasites & Vectors

Figure 4

From: Molecular identification of Trichinella spiralis nudix hydrolase and its induced protective immunity against trichinellosis in BALB/c mice

Figure 4

Identification of recombinant TsNd protein. (A) The SDS-PAGE analysis of T. spiralis ML crude antigens (lane 1), ES antigens (lane 2), and rTsNd. (B) Western blot analysis of rTsNd antigenicity. The T. spiralis ML crude proteins (lane 1), ES proteins (lane 2), and rTsNd (lane 3) were recognized by sera of mice infected with T. spiralis at 42 dpi. The native TsNd protein in crude antigens (lane 4), ES antigens (lane 5), and rTsNd (lane 6) were recognized by anti-rTsNd serum. The T. spiralis crude proteins (lane 7), ES proteins (lane 8), and rTsNd (lane 9) were not recognized by sera of normal mice. (C) Western blot analysis of the crude antigens of T. spiralis different developmental stages by using anti-rTsNd serum. The native TsNd protein in crude antigens of T. spiralis IIL at 6 hpi (lane 1), AW at 3 dpi (lane 2), and NBL (lane 3) were recognized by anti-rTsNd serum.

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