Figure 1

Leukocyte recruitment in response to L. longipalpis SGS during L. infantum infection. C57BL/6 mice were injected i.p. with saline (control), L. infantum and/or SGS as described in methods. One, 3 and 6 hours after stimulation, peritoneal cavities were washed and cells were harvested. Kinetics of total leucocytes (A), monocytes (B) and neutrophils (C) were estimated using Diff-Quick-stained cytospin preparations. Area Under Curve (AUC) of the kinetic of total leukocytes (D), monocytes (E) and neutrophils (F) recruitment. The data are the means and SEM from an experiment representative of three independent experiments. n = 5 per group. (*, p < 0.05 and Mann–Whitney test, two-tailed).